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WB analysis of 293 whole cell lysate using GTX01525 ALKBH5 antibody. Dilution : 1:2000 Loading : 20μg per lane
WB analysis of 293 whole cell lysate using GTX01525 ALKBH5 antibody. Dilution : 1:2000 Loading : 20μg per lane
WB analysis of 293 whole cell lysate using GTX01525 ALKBH5 antibody. Dilution : 1:2000 Loading : 20μg per lane

ALKBH5 antibody

GTX01525
GeneTex
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetALKBH5
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Overview

  • Supplier
    GeneTex
  • Product Name
    ALKBH5 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500. IHC-P: 1:100. FCM: 1:25. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54890
  • Target name
    ALKBH5
  • Target description
    alkB homolog 5, RNA demethylase
  • Target synonyms
    ABH5, OFOXD, OFOXD1, RNA demethylase ALKBH5, AlkB family member 5, RNA demethylase, alkB, alkylation repair homolog 5, alkylated DNA repair protein alkB homolog 5, alpha-ketoglutarate-dependent dioxygenase alkB homolog 5, oxoglutarate and iron-dependent oxygenase domain containing, probable alpha-ketoglutarate-dependent dioxygenase ABH5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6P6C2
  • Protein Name
    RNA demethylase ALKBH5
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ALKBH5 using anti-ALKBH5 antibody (A03360). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human U87 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALKBH5 antigen affinity purified polyclonal antibody (Catalog # A03360) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALKBH5 at approximately 52 kDa. The expected band size for ALKBH5 is at 44 kDa.
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