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Western Blot Positive WB detected in: K562 whole cell lysate, SH-SY5Y whole cell lysate All lanes: ALOX12 antibody at 3.53microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 76 kDa Observed band size: 76 kDa
Western Blot Positive WB detected in: K562 whole cell lysate, SH-SY5Y whole cell lysate All lanes: ALOX12 antibody at 3.53microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 76 kDa Observed band size: 76 kDa
Western Blot Positive WB detected in: K562 whole cell lysate, SH-SY5Y whole cell lysate All lanes: ALOX12 antibody at 3.53microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 76 kDa Observed band size: 76 kDa

ALOX12 Antibody

CSB-PA001617LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetALOX12
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Overview

  • Supplier
    Cusabio
  • Product Name
    ALOX12 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID239
  • Target name
    ALOX12
  • Target description
    arachidonate 12-lipoxygenase, 12S type
  • Target synonyms
    12-LOX, 12S-LOX, LOG12, polyunsaturated fatty acid lipoxygenase ALOX12, 12S-lipoxygenase, Arachidonate 12-lipoxygenase, 12S-type, arachidonate (12S)-lipoxygenase, arachidonate (15S)-lipoxygenase, arachidonate 15-lipoxygenase,15S-type, linoleate 13S-lipoxygenase, lipoxin synthase 12-LO, platelet 12-LOX, platelet-type 12-lipoxygenase, platelet-type lipoxygenase 12
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP18054
  • Protein Name
    Arachidonate 12-lipoxygenase, 12S-type
  • Scientific Description
    Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12S)-hydroperoxyeicosatetraenoic acid/(12S)-HPETE but can also metabolize linoleic acid. Has a dual activity since it also converts leukotriene A4/LTA4 into both the bioactive lipoxin A4/LXA4 and lipoxin B4/LXB4. Through the production of specific bioactive lipids like (12S)-HPETE it regulates different biological processes including platelet activation. It also probably positively regulates angiogenesis through regulation of the expression of the vascular endothelial growth factor. Plays a role in apoptotic process, promoting the survival of vascular smooth muscle cells for instance. May also play a role in the control of cell migration and proliferation.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of 12 Lipoxygenase using anti-12 Lipoxygenase antibody (A02275-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: rat spleen tissue lysates, lane 2: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-12 Lipoxygenase antigen affinity purified polyclonal antibody (Catalog # A02275-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 12 Lipoxygenase at approximately 80KD. The expected band size for 12 Lipoxygenase is at 75-80KD.
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