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ALOX12 antibody

GTX130257
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetALOX12
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Overview

  • Supplier
    GeneTex
  • Product Name
    ALOX12 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    2.96 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID239
  • Target name
    ALOX12
  • Target description
    arachidonate 12-lipoxygenase, 12S type
  • Target synonyms
    12-LOX; 12S-lipoxygenase; 12S-LOX; arachidonate (12S)-lipoxygenase; arachidonate (15S)-lipoxygenase; Arachidonate 12-lipoxygenase, 12S-type; arachidonate 15-lipoxygenase,15S-type; linoleate 13S-lipoxygenase; lipoxin synthase 12-LO; LOG12; platelet 12-LOX; platelet-type 12-lipoxygenase; platelet-type lipoxygenase 12; polyunsaturated fatty acid lipoxygenase ALOX12
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP18054
  • Protein Name
    Arachidonate 12-lipoxygenase, 12S-type
  • Scientific Description
    Oxygenase and 14,15-leukotriene A4 synthase activity.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of 12 Lipoxygenase using anti-12 Lipoxygenase antibody (A02275-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: rat spleen tissue lysates, lane 2: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-12 Lipoxygenase antigen affinity purified polyclonal antibody (Catalog # A02275-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 12 Lipoxygenase at approximately 80KD. The expected band size for 12 Lipoxygenase is at 75-80KD.
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