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ALOX12 Polyclonal Antibody

BS-3874R
Bioss
ApplicationsImmunoFluorescence, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetALOX12
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Overview

  • Supplier
    Bioss
  • Product Name
    ALOX12/12 Lipoxygenase Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC-P(1:200-400), IHC-F(1:100-500), IF()
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID239
  • Target name
    ALOX12
  • Target description
    arachidonate 12-lipoxygenase, 12S type
  • Target synonyms
    12-LOX, 12S-LOX, LOG12, polyunsaturated fatty acid lipoxygenase ALOX12, 12S-lipoxygenase, Arachidonate 12-lipoxygenase, 12S-type, arachidonate (12S)-lipoxygenase, arachidonate (15S)-lipoxygenase, arachidonate 15-lipoxygenase,15S-type, linoleate 13S-lipoxygenase, lipoxin synthase 12-LO, platelet 12-LOX, platelet-type 12-lipoxygenase, platelet-type lipoxygenase 12
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of 12 Lipoxygenase using anti-12 Lipoxygenase antibody (A02275-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: rat spleen tissue lysates, lane 2: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-12 Lipoxygenase antigen affinity purified polyclonal antibody (Catalog # A02275-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 12 Lipoxygenase at approximately 80KD. The expected band size for 12 Lipoxygenase is at 75-80KD.
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