FACS analysis of 70% ethanol fixed HepG2 cells using GTX15777 alpha 1 Antitrypsin antibody [TMF1#4B5]. Red histogram : Primary antibody Pink histogram : mouse isotype control Fixation : 70% ethanol for 10 minutes Permeabilization : 0.25% Trito X-100 for 20 minutes Dilution : 3-5 microg/million cells in 2.5% BSA for 30 minutes at room temperature
![WB analysis of membrane enriched extracts (30 ug lysate) of Hep G2 (Lane 1) and tissue extract of Mouse Liver (Lane 2) using GTX15777 alpha 1 Antitrypsin antibody [TMF1#4B5]. Dilution : 2 microg/ml](https://www.genetex.com/upload/website/prouct_img/normal/GTX15777/GTX15777_1529_WB_w_23060620_134.webp "WB analysis of membrane enriched extracts (30 ug lysate) of Hep G2 (Lane 1) and tissue extract of Mouse Liver (Lane 2) using GTX15777 alpha 1 Antitrypsin antibody [TMF1#4B5]. Dilution : 2 microg/ml")
![WB analysis of membrane enriched extracts (30 ug) of HT-29 (Lane 1) and Hep G2 (Lane 2) in figure 1, and condition media of Hep G2 (Lane 1), PC3 (Lane 2), HeLa (Lane 3) in figure 2 using GTX15777 alpha 1 Antitrypsin antibody [TMF1#4B5]. Dilution : 2 microg/ml](https://www.genetex.com/upload/website/prouct_img/normal/GTX15777/GTX15777_1530_WB_w_23060620_605.webp "WB analysis of membrane enriched extracts (30 ug) of HT-29 (Lane 1) and Hep G2 (Lane 2) in figure 1, and condition media of Hep G2 (Lane 1), PC3 (Lane 2), HeLa (Lane 3) in figure 2 using GTX15777 alpha 1 Antitrypsin antibody [TMF1#4B5]. Dilution : 2 microg/ml")
FACS analysis of 70% ethanol fixed HepG2 cells using GTX15777 alpha 1 Antitrypsin antibody [TMF1#4B5]. Red histogram : Primary antibody Pink histogram : mouse isotype control Fixation : 70% ethanol for 10 minutes Permeabilization : 0.25% Trito X-100 for 20 minutes Dilution : 3-5 microg/million cells in 2.5% BSA for 30 minutes at room temperature
alpha 1 Antitrypsin antibody [TMF1#4B5]
GTX15777
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ELISA, RadioImmunoAssay
Product group Antibodies
ReactivityHuman, Mouse
TargetSERPINA1
Overview
- SupplierGeneTex
- Product Namealpha 1 Antitrypsin antibody [TMF1#4B5]
- Delivery Days Customer9
- Application Supplier NoteWB: 1-3 microg/ml. FACS: 3-5 microg/106 cells. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ELISA, RadioImmunoAssay
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDTMF1#4B5
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5265
- Target nameSERPINA1
- Target descriptionserpin family A member 1
- Target synonymsA1A; A1AT; AAT; alpha-1 antitrypsin; alpha-1 protease inhibitor; alpha-1 proteinase inhibitor; alpha-1-antiproteinase; alpha-1-antitrypsin; alpha-1-antitrypsin short transcript variant 1C4; alpha-1-antitrypsin short transcript variant 1C5; alpha1AT; anti-elastase; epididymis secretory sperm binding protein; nNIF; PI; PI1; PRO2275; protease inhibitor 1 (anti-elastase), alpha-1-antitrypsin; serine (or cysteine) proteinase inhibitor, clade A, member 1; serpin A1; serpin peptidase inhibitor clade A (alpha-1antiproteinase, antitrypsin) member 1; serpin peptidase inhibitor clade A member 1; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
- HostMouse
- IsotypeIgG2a
- Protein IDP01009
- Protein NameAlpha-1-antitrypsin
- Scientific DescriptionThe protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203