IHC-P analysis of human breast adenocarcinoma tissue using GTX84661 alpha B Crystallin antibody [3C2]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
![IHC-P analysis of human colon adenocarcinoma tissue using GTX84661 alpha B Crystallin antibody [3C2]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84661/GTX84661_2997_IHC-P_w_23061420_219.webp "IHC-P analysis of human colon adenocarcinoma tissue using GTX84661 alpha B Crystallin antibody [3C2]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human ovary adenocarcinoma tissue using GTX84661 alpha B Crystallin antibody [3C2]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84661/GTX84661_2998_IHC-P_w_23061420_919.webp "IHC-P analysis of human ovary adenocarcinoma tissue using GTX84661 alpha B Crystallin antibody [3C2]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human bladder carcinoma tissue using GTX84661 alpha B Crystallin antibody [3C2]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84661/GTX84661_2999_IHC-P_w_23061420_713.webp "IHC-P analysis of human bladder carcinoma tissue using GTX84661 alpha B Crystallin antibody [3C2]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human pancreas carcinoma tissue using GTX84661 alpha B Crystallin antibody [3C2]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84661/GTX84661_3000_IHC-P_w_23061420_624.webp "IHC-P analysis of human pancreas carcinoma tissue using GTX84661 alpha B Crystallin antibody [3C2]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human lymphoma tissue using GTX84661 alpha B Crystallin antibody [3C2]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84661/GTX84661_3001_IHC-P_w_23061420_882.webp "IHC-P analysis of human lymphoma tissue using GTX84661 alpha B Crystallin antibody [3C2]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IP analysis of DDDDK tagged alpha B Crystallin overexpressed HEK293T lysate using GTX84661 alpha B Crystallin antibody [3C2].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate](https://www.genetex.com/upload/website/prouct_img/normal/GTX84661/GTX84661_3333_IP_w_23061420_189.webp "IP analysis of DDDDK tagged alpha B Crystallin overexpressed HEK293T lysate using GTX84661 alpha B Crystallin antibody [3C2].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate")
![WB analysis of A549 and COS-7 cell lysate using GTX84661 alpha B Crystallin antibody [3C2]. Loading : 10 ug per lane Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX84661/GTX84661_3556_WB_w_23061420_322.webp "WB analysis of A549 and COS-7 cell lysate using GTX84661 alpha B Crystallin antibody [3C2]. Loading : 10 ug per lane Dilution : 1:200")
![WB analysis of HEK293T cells transfected with alpha B Crystallin plasmid (Right) or empty vector (Left) for 48 hrs using GTX84661 alpha B Crystallin antibody [3C2]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84661/GTX84661_4539_WB_w_23061420_819.webp "WB analysis of HEK293T cells transfected with alpha B Crystallin plasmid (Right) or empty vector (Left) for 48 hrs using GTX84661 alpha B Crystallin antibody [3C2]. Loading : 5 ug per lane")
![FACS analysis of HEK293T cells transfected with either alpha B Crystallin plasmid(Red) or empty vector control plasmid(Blue) using GTX84661 alpha B Crystallin antibody [3C2].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84661/GTX84661_495_FACS_w_23061420_663.webp "FACS analysis of HEK293T cells transfected with either alpha B Crystallin plasmid(Red) or empty vector control plasmid(Blue) using GTX84661 alpha B Crystallin antibody [3C2].")
IHC-P analysis of human breast adenocarcinoma tissue using GTX84661 alpha B Crystallin antibody [3C2]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
alpha B Crystallin antibody [3C2]
GTX84661
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Monkey
TargetCRYAB
Overview
- SupplierGeneTex
- Product Namealpha B Crystallin antibody [3C2] - Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:5000. IHC-P: 1:50. FACS: 1:100. IP: 2ug/500ul. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID3C2
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID1410
- Target nameCRYAB
- Target descriptioncrystallin alpha B
- Target synonymsalpha-crystallin B chain; CMD1II; CRYA2; CTPP2; CTRCT16; epididymis secretory protein Li 101; heat shock protein beta-5; heat-shock 20 kD like-protein; HEL-S-101; HSPB5; MFM2; renal carcinoma antigen NY-REN-27; rosenthal fiber component
- HostMouse
- IsotypeIgG1
- Protein IDP02511
- Protein NameAlpha-crystallin B chain
- Scientific DescriptionMay contribute to the transparency and refractive index of the lens.
- ReactivityHuman, Monkey
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203