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Western Blot Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate All lanes: AMD1 antibody at 2.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 39, 22 kDa Observed band size: 39 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate All lanes: AMD1 antibody at 2.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 39, 22 kDa Observed band size: 39 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate All lanes: AMD1 antibody at 2.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 39, 22 kDa Observed band size: 39 kDa

AMD1 Antibody

CSB-PA001568LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetAMD1
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Overview

  • Supplier
    Cusabio
  • Product Name
    AMD1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID262
  • Target name
    AMD1
  • Target description
    adenosylmethionine decarboxylase 1
  • Target synonyms
    ADOMETDC, AMD, SAMDC, S-adenosylmethionine decarboxylase proenzyme, S-adenosylmethionine decarboxylase 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP17707
  • Protein Name
    S-adenosylmethionine decarboxylase proenzyme
  • Scientific Description
    Essential for biosynthesis of the polyamines spermidine and spermine. Promotes maintenance and self-renewal of embryonic stem cells, by maintaining spermine levels (By similarity).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of AMD1 using anti-AMD1 antibody (A06146). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMD1 antigen affinity purified polyclonal antibody (Catalog # A06146) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMD1 at approximately 38KD. The expected band size for AMD1 is at 38KD.
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