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Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-SAMDC/AMD1 Polyclonal Antibody, Unconjugated (bs-1887r) at 1:200. The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-SAMDC/AMD1 Polyclonal Antibody, Unconjugated (bs-1887r) at 1:200. The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-SAMDC/AMD1 Polyclonal Antibody, Unconjugated (bs-1887r) at 1:200. The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.

AMD1/SAMDC Polyclonal Antibody

BS-1887R
Bioss
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat
TargetAMD1
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Overview

  • Supplier
    Bioss
  • Product Name
    AMD1/SAMDC Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID262
  • Target name
    AMD1
  • Target description
    adenosylmethionine decarboxylase 1
  • Target synonyms
    ADOMETDC, AMD, SAMDC, S-adenosylmethionine decarboxylase proenzyme, S-adenosylmethionine decarboxylase 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP17707
  • Protein Name
    S-adenosylmethionine decarboxylase proenzyme
  • Reactivity
    Bovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of AMD1 using anti-AMD1 antibody (A06146). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMD1 antigen affinity purified polyclonal antibody (Catalog # A06146) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMD1 at approximately 38KD. The expected band size for AMD1 is at 38KD.
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