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ProductsBack/AMOTL2 antibody [N3C2], Internal

AMOTL2 antibody [N3C2], Internal

GTX120712
GeneTex
ApplicationsImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetAMOTL2
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Overview

  • Supplier
    GeneTex
  • Product Name
    AMOTL2 antibody [N3C2], Internal
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.02 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID51421
  • Target name
    AMOTL2
  • Target description
    angiomotin like 2
  • Target synonyms
    LCCP, angiomotin-like protein 2, Leman coiled-coil protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y2J4
  • Protein Name
    Angiomotin-like protein 2
  • Scientific Description
    Angiomotin is a protein that binds angiostatin, a circulating inhibitor of the formation of new blood vessels (angiogenesis). Angiomotin mediates angiostatin inhibition of endothelial cell migration and tube formation in vitro. The protein encoded by this gene is related to angiomotin and is a member of the motins protein family. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Eder N, Roncaroli F, Domart MC, et al. YAP1/TAZ drives ependymoma-like tumour formation in mice. Nat Commun. 2020,11(1):2380. doi: 10.1038/s41467-020-16167-y
    Read this paper
  • Hildebrand S, Hultin S, Subramani A, et al. The E-cadherin/AmotL2 complex organizes actin filaments required for epithelial hexagonal packing and blastocyst hatching. Sci Rep. 2017,7(1):9540. doi: 10.1038/s41598-017-10102-w
    Read this paper
  • Proszynski TJ, Sanes JR. Amotl2 interacts with LL5β, localizes to podosomes and regulates postsynaptic differentiation in muscle. J Cell Sci. 2013,126(Pt 10):2225-35. doi: 10.1242/jcs.121327
    Read this paper

Datasheet

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Figure 1. Western blot analysis of AMOTL2 using anti-AMOTL2 antibody (A06852). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMOTL2 antigen affinity purified polyclonal antibody (Catalog # A06852) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMOTL2 at approximately 95KD. The expected band size for AMOTL2 is at 86KD.
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