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IHC-P analysis of human thyroid gland tissue using GTX86973 AMPD1 antibody. The picture on the right is blocked with the synthesized peptide.
IHC-P analysis of human thyroid gland tissue using GTX86973 AMPD1 antibody. The picture on the right is blocked with the synthesized peptide.
IHC-P analysis of human thyroid gland tissue using GTX86973 AMPD1 antibody. The picture on the right is blocked with the synthesized peptide.

AMPD1 antibody

GTX86973
GeneTex
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetAMPD1
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Overview

  • Supplier
    GeneTex
  • Product Name
    AMPD1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 1:50~1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID270
  • Target name
    AMPD1
  • Target description
    adenosine monophosphate deaminase 1
  • Target synonyms
    adenosine monophosphate deaminase 1 (isoform M); adenosine monophosphate deaminase-1 (muscle); AMP deaminase 1; AMPD; MAD; MADA; MMDD; myoadenylate deaminase; skeletal muscle AMPD
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP23109
  • Protein Name
    AMP deaminase 1
  • Scientific Description
    Adenosine monophosphate deaminase 1 catalyzes the deamination of AMP to IMP in skeletal muscle and plays an important role in the purine nucleotide cycle. Two other genes have been identified, AMPD2 and AMPD3, for the liver- and erythocyte-specific isoforms, respectively. Deficiency of the muscle-specific enzyme is apparently a common cause of exercise-induced myopathy and probably the most common cause of metabolic myopathy in the human. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Feb 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AMPD1 using anti-AMPD1 antibody (A04209-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: rat skeletal muscle tissue lysates, Lane 5: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMPD1 antigen affinity purified polyclonal antibody (Catalog # A04209-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMPD1 at approximately 86 kDa. The expected band size for AMPD1 is at 86 kDa.
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