![ELISA analysis of antigen using GTX60403 AMPK alpha 1 antibody [2B7]. Red : Control antigen 100ng Purple : Antigen 10ng Green : Antigen 50ng Blue : Antigen 100ng ELISA analysis of antigen using GTX60403 AMPK alpha 1 antibody [2B7]. Red : Control antigen 100ng Purple : Antigen 10ng Green : Antigen 50ng Blue : Antigen 100ng](https://www.genetex.com/upload/website/prouct_img/normal/GTX60403/GTX60403_20170912_ELISA_w_23061123_331.webp)
ELISA analysis of antigen using GTX60403 AMPK alpha 1 antibody [2B7]. Red : Control antigen 100ng Purple : Antigen 10ng Green : Antigen 50ng Blue : Antigen 100ng
AMPK alpha 1 antibody [2B7]
GTX60403
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPRKAA1
Overview
- SupplierGeneTex
- Product NameAMPK alpha 1 antibody [2B7]
- Delivery Days Customer9
- Application Supplier NoteWB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. IHC-P: 1/200 - 1/1000. FACS: 1/200 - 1/400. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID2B7
- ConjugateUnconjugated
- Gene ID5562
- Target namePRKAA1
- Target descriptionprotein kinase AMP-activated catalytic subunit alpha 1
- Target synonymsAMPK, AMPK alpha 1, AMPKa1, 5'-AMP-activated protein kinase catalytic subunit alpha-1, 5'-AMP-activated protein kinase, catalytic alpha-1 chain, ACACA kinase, AMP -activate kinase alpha 1 subunit, AMP-activated protein kinase, catalytic, alpha-1, AMPK subunit alpha-1, AMPK, alpha, 1, HMGCR kinase, acetyl-CoA carboxylase kinase, hydroxymethylglutaryl-CoA reductase kinase, protein kinase, AMP-activated, alpha 1 catalytic subunit, tau-protein kinase PRKAA1
- HostMouse
- IsotypeIgG1
- Protein IDQ13131
- Protein Name5'-AMP-activated protein kinase catalytic subunit alpha-1
- Scientific DescriptionThe protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Boosting mitochondrial function and metabolism in aging female germ cells with dual ROCK/ROS inhibition.Read more
- Metformin protects against PM2.5-induced lung injury and cardiac dysfunction independent of AMP-activated protein kinase alpha2. Gao J et al., 2020 Jan, Redox BiolRead more
- AMPKalpha2 deficiency exacerbates long-term PM2.5 exposure-induced lung injury and cardiac dysfunction. Wang H et al., 2018 Jun, Free Radic Biol MedRead more
![FACS analysis of PC-2 cells using GTX60403 AMPK alpha 1 antibody [2B7]. Green : AMPK alpha 1 Purple : negative control FACS analysis of PC-2 cells using GTX60403 AMPK alpha 1 antibody [2B7]. Green : AMPK alpha 1 Purple : negative control](https://www.genetex.com/upload/website/prouct_img/normal/GTX60403/GTX60403_20170912_FACS_w_23061123_748.webp)
![ICC/IF analysis of NTERA-2 cells using GTX60403 AMPK alpha 1 antibody [2B7]. Green : AMPK alpha 1 Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments ICC/IF analysis of NTERA-2 cells using GTX60403 AMPK alpha 1 antibody [2B7]. Green : AMPK alpha 1 Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments](https://www.genetex.com/upload/website/prouct_img/normal/GTX60403/GTX60403_20170912_ICCIF_w_23061123_524.webp)
![IHC-P analysis of ovarian cancer (left) and brain tissue (right) using GTX60403 AMPK alpha 1 antibody [2B7]. IHC-P analysis of ovarian cancer (left) and brain tissue (right) using GTX60403 AMPK alpha 1 antibody [2B7].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60403/GTX60403_20170912_IHC-P_w_23061123_752.webp)
![WB analysis of Jurkat (1), Hela (2), HepG2 (3), MCF-7 (4), Cos7 (5), NIH-3T3 (6), K562 (7), HEK293 (8), and PC-12 (9) cell lysate using GTX60403 AMPK alpha 1 antibody [2B7]. WB analysis of Jurkat (1), Hela (2), HepG2 (3), MCF-7 (4), Cos7 (5), NIH-3T3 (6), K562 (7), HEK293 (8), and PC-12 (9) cell lysate using GTX60403 AMPK alpha 1 antibody [2B7].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60403/GTX60403_20170912_WB_w_23061123_351.webp)



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