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Immunofluorescence analysis of HepG2 cells, using Cyclosome 1 antibody.
Immunofluorescence analysis of HepG2 cells, using Cyclosome 1 antibody.
Immunofluorescence analysis of HepG2 cells, using Cyclosome 1 antibody.

ANAPC1 Antibody

CSB-PA549756
Cusabio
ApplicationsImmunoFluorescence, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetANAPC1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ANAPC1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID64682
  • Target name
    ANAPC1
  • Target description
    anaphase promoting complex subunit 1
  • Target synonyms
    APC1, MCPR, TSG24, anaphase-promoting complex subunit 1, anaphase-promoting complex 1 (meiotic checkpoint regulator), cyclosome subunit 1, mitotic checkpoint regulator, testis-specific gene 24 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H1A4
  • Protein Name
    Anaphase-promoting complex subunit 1
  • Scientific Description
    Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of Lys-11-linked polyubiquitin chains and, to a lower extent, the formation of Lys-48- and Lys-63-linked polyubiquitin chains. Joergensen P.M., Gene 262:51-59(2001). Ota T., Nat. Genet. 36:40-45(2004). Kraft C., EMBO J. 22:6598-6609(2003).
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of APC1/ANAPC1 using anti-APC1/ANAPC1 antibody (A03471-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APC1/ANAPC1 antigen affinity purified polyclonal antibody (Catalog # A03471-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APC1/ANAPC1 at approximately 240 kDa. The expected band size for APC1/ANAPC1 is at 217 kDa.
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