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Androgen receptor Antibody (YA620, Rabbit)

HY-P80014
MedChem Express
TargetAR
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Androgen receptor Antibody (YA620, Rabbit)
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID367
  • Target name
    AR
  • Target description
    androgen receptor
  • Target synonyms
    AIS; androgen receptor; AR8; DHTR; dihydrotestosterone receptor; HUMARA; HYSP1; KD; NR3C4; nuclear receptor subfamily 3 group C member 4; SBMA; SMAX1; TFM
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Androgen receptor Antibody (YA620) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Androgen receptor.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Androgen Receptor/AR using anti-Androgen Receptor/AR antibody (A00542). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human 22RV1 whole cell lysates, Lane 6: human HepG2 whole cell lysates, Lane 7: human CACO-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Androgen Receptor/AR antigen affinity purified polyclonal antibody (Catalog # A00542) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Androgen Receptor/AR at approximately 120KD. The expected band size for Androgen Receptor/AR is at 120KD.
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