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ProductsBack/Angiotensin Converting Enzyme 1 (ACE) Rabbit Polyclonal Antibody
Western blot (WB) analysis of ACE1 antibody (Cat.-No.: AP20608PU-N) in extracts from mouse kidney cells.
Western blot (WB) analysis of ACE1 antibody (Cat.-No.: AP20608PU-N) in extracts from mouse kidney cells.
Western blot (WB) analysis of ACE1 antibody (Cat.-No.: AP20608PU-N) in extracts from mouse kidney cells.

Angiotensin Converting Enzyme 1 (ACE) Rabbit Polyclonal Antibody

AP20608PU-N
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetACE
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Angiotensin Converting Enzyme 1 (ACE) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID1636
  • Target name
    ACE
  • Target description
    angiotensin I converting enzyme
  • Target synonyms
    ACE1, CD143, DCP, DCP1, angiotensin-converting enzyme, CD143 antigen, angiotensin I converting enzyme (peptidyl-dipeptidase A) 1, carboxycathepsin, dipeptidyl carboxypeptidase 1, dipeptidyl carboxypeptidase I, kininase II, peptidase P
  • Host
    Rabbit
  • Protein IDP12821
  • Protein Name
    Angiotensin-converting enzyme
  • Scientific Description
    Angiotensin Converting Enzyme 1 (ACE) rabbit polyclonal antibody, Aff - Purified
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ACE using anti-ACE antibody (A00251-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse lung tissue lysates, Lane 3: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACE antigen affinity purified polyclonal antibody (Catalog # A00251-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACE at approximately 150-180 kDa. The expected band size for ACE is at 150 kDa.
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Anti-ACE Antibody Picoband(r)A00251-3-CARRIER-FREE
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ReactivityHuman, Mouse, Rat
TargetACE
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Product group Antibodies
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Angiotensin I Converting Enzyme (ACE) Polyclonal AntibodyCAU29038
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TargetACE
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Formalin-fixed and paraffin embedded rat pancreas tissue labeled with Anti-ACE1 Polyclonal Antibody, Unconjugated (bs-0439R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Product group Antibodies
References
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TargetACE
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TargetACE
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Atlas Antibodies
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ACE antibody detects ACE protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. ACE stained by ACE antibody (GTX130534) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GeneTex
ACE antibodyGTX130534
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetACE
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