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Annexin VI Antibody (YA618, Rabbit)

HY-P80945
MedChem Express
TargetANXA6
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Annexin VI Antibody (YA618, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID309
  • Target name
    ANXA6
  • Target description
    annexin A6
  • Target synonyms
    ANX6, CBP68, CPB-II, p68, p70, annexin A6, 67 kDa calelectrin, annexin VI (p68), annexin-6, calcium-binding protein p68, calelectrin, calphobindin II, chromobindin-20, lipocortin VI, testis secretory sperm-binding protein Li 198a
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Annexin VI Antibody (YA618) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Annexin VI.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Annexin VI/ANXA6 using anti-Annexin VI/ANXA6 antibody (A03735). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Annexin VI/ANXA6 antigen affinity purified polyclonal antibody (Catalog # A03735) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Annexin VI/ANXA6 at approximately 68-76 kDa. The expected band size for Annexin VI/ANXA6 is at 76 kDa.
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