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ProductsBack/ANXA6/Annexin A6/Annexin VI Antibody

ANXA6/Annexin A6/Annexin VI Antibody

LS-C764551
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetANXA6
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ANXA6/Annexin A6/Annexin VI Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:100 - 1:400), WB (1:1000 - 1:4000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID309
  • Target name
    ANXA6
  • Target description
    annexin A6
  • Target synonyms
    ANX6, CBP68, CPB-II, p68, p70, annexin A6, 67 kDa calelectrin, annexin VI (p68), annexin-6, calcium-binding protein p68, calelectrin, calphobindin II, chromobindin-20, lipocortin VI, testis secretory sperm-binding protein Li 198a
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Annexin VI/ANXA6 using anti-Annexin VI/ANXA6 antibody (A03735). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Annexin VI/ANXA6 antigen affinity purified polyclonal antibody (Catalog # A03735) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Annexin VI/ANXA6 at approximately 68-76 kDa. The expected band size for Annexin VI/ANXA6 is at 76 kDa.
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