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ProductsBack/ANO1 / DOG1 / TMEM16A Antibody

ANO1 / DOG1 / TMEM16A Antibody

LS-C497208
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetANO1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ANO1 / DOG1 / TMEM16A Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 74kDa/97kDa/114kDa, while the observed MW by Western blot was 114kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:1000 - 1:2000) The predicted MW is 74kDa/97kDa/114kDa, while the observed MW by Western blot was 114kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    4.63 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID55107
  • Target name
    ANO1
  • Target description
    anoctamin 1
  • Target synonyms
    anoctamin 1, calcium activated chloride channel; anoctamin-1; Ca2+-activated Cl- channel; calcium activated chloride channel; discovered on gastrointestinal stromal tumors protein 1; DOG1; oral cancer overexpressed 2; ORAOV2; TAOS2; TMEM16A; transmembrane protein 16A (eight membrane-spanning domains); tumor-amplified and overexpressed sequence 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Lane 1: Mouse Skeletal muscle lysates; Lane 2: Rat Skeletal muscle lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human A673 cell lysates; Lane 5: Human U-2OS cell lysates; Lane 6: Human MKN45 cell lysates probed with TMEM16A Polyclonal Antibody, Unconjugated (bs-3794R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C
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Figure 1. Western blot analysis of TMEM16A using anti-TMEM16A antibody (A00713). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate, Lane 2: human HepG2 whole cell lysate, Lane 3: human A549 whole cell lysate, Lane 4: human PANC-1 whole cell lysate, Lane 5: human SK-OV-3 whole cell lysate, Lane 6: human SGC-7901 whole cell lysate, Lane 7: human COLO-320 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM16A antigen affinity purified polyclonal antibody (Catalog # A00713) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM16A at approximately 130KD. The expected band size for TMEM16A is at 114KD.
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