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Anti-DOG1 [19F2]

Ab03308-10.0
Absolute Antibody
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetANO1
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-DOG1 [19F2]
  • Delivery Days Customer
    7
  • Application Supplier Note
    The antibody 19F2 and the commercial antibody SP31 were used to stain breast tissue and gastrointestinal tumor in immunohistochemistry. Results showed the antibody specificity is equivalent to the commercial antibody. Further, the staining intensity of the antibody 19F2 was higher than the commercial antibody (CN113045652A).
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    IHC; WB; ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    19F2
  • Gene ID55107
  • Target name
    ANO1
  • Target description
    anoctamin 1
  • Target synonyms
    anoctamin 1, calcium activated chloride channel; anoctamin-1; Ca2+-activated Cl- channel; calcium activated chloride channel; discovered on gastrointestinal stromal tumors protein 1; DOG1; oral cancer overexpressed 2; ORAOV2; TAOS2; TMEM16A; transmembrane protein 16A (eight membrane-spanning domains); tumor-amplified and overexpressed sequence 2
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDQ5XXA6
  • Protein Name
    Anoctamin-1
  • Scientific Description
    This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    The original antibody was generated by immunizing mice with recombinant protein DOG1.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Lane 1: Mouse Skeletal muscle lysates; Lane 2: Rat Skeletal muscle lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human A673 cell lysates; Lane 5: Human U-2OS cell lysates; Lane 6: Human MKN45 cell lysates probed with TMEM16A Polyclonal Antibody, Unconjugated (bs-3794R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C
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Figure 1. Western blot analysis of TMEM16A using anti-TMEM16A antibody (A00713). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate, Lane 2: human HepG2 whole cell lysate, Lane 3: human A549 whole cell lysate, Lane 4: human PANC-1 whole cell lysate, Lane 5: human SK-OV-3 whole cell lysate, Lane 6: human SGC-7901 whole cell lysate, Lane 7: human COLO-320 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM16A antigen affinity purified polyclonal antibody (Catalog # A00713) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM16A at approximately 130KD. The expected band size for TMEM16A is at 114KD.
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