ProductsBack/Anti-SSH3BP1/ABI1 Antibody Picoband(r)

Figure 1. Western blot analysis of SSH3BP1/ABI1 using anti-SSH3BP1/ABI1 antibody (PB9416). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human U87 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SSH3BP1/ABI1 antigen affinity purified polyclonal antibody (Catalog # PB9416) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SSH3BP1/ABI1 at approximately 65 kDa. The expected band size for SSH3BP1/ABI1 is at 55 kDa.

Anti-SSH3BP1/ABI1 Antibody Picoband(r)

PB9416

ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry

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Product group Antibodies

TargetABI1

Overview

Supplier
Boster Bio
Product Name
Anti-ABI1 Picoband Antibody
Delivery Days Customer
9
Application Supplier Note
WB: The detection limit for ABI1 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
Applications
Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Certification
Research Use Only
Clonality
Polyclonal
Concentration
500 ug/ml
Gene ID10006
Target name
ABI1
Target description
abl interactor 1
Target synonyms
Abelson interactor 1; ABI-1; abl interactor 1; abl-binding protein 4; ABLBP4; Abl-interactor protein 1 long; E3B1; eps8 SH3 domain-binding protein; interactor protein AblBP4; nap1 binding protein; NAP1BP; spectrin SH3 domain-binding protein 1; SSH3BP; SSH3BP1
Host
Rabbit
Isotype
IgG
Protein IDQ8IZP0
Protein Name
Abl interactor 1
Scientific Description
Boster Bio Anti-SSH3BP1/ABI1 Antibody Picoband® catalog # PB9416. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage Instruction
-20°C,2°C to 8°C
UNSPSC
12352203