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Immunohistochemical staining of human breast shows moderate positivity in cytoplasm in glandular cells.
Immunohistochemical staining of human breast shows moderate positivity in cytoplasm in glandular cells.
Immunohistochemical staining of human breast shows moderate positivity in cytoplasm in glandular cells.

Anti-ACACA Antibody

HPA063018
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetACACA
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ACACA Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID31
  • Target name
    ACACA
  • Target description
    acetyl-CoA carboxylase alpha
  • Target synonyms
    ACAC, ACACAD, ACACalpha, ACC, ACC1, ACCA, ACCalpha, Acac1, hACC1, acetyl-CoA carboxylase 1, ACC-alpha, acetyl-Coenzyme A carboxylase alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13085
  • Protein Name
    Acetyl-CoA carboxylase 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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FAQ

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Figure 1. Western blot analysis of ACACA using anti-ACACA antibody (A01802-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACACA antigen affinity purified polyclonal antibody (Catalog # A01802-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACACA at approximately 266 kDa. The expected band size for ACACA is at 266 kDa.
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