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Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.

Anti-ACKR3 Antibody

HPA049718
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
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Product group Antibodies
TargetACKR3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ACKR3
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57007
  • Target name
    ACKR3
  • Target description
    atypical chemokine receptor 3
  • Target synonyms
    atypical chemokine receptor 3; chemokine (C-X-C motif) receptor 7; chemokine orphan receptor 1; CMKOR1; C-X-C chemokine receptor type 7; CXCR7; CXC-R7; CXCR-7; G protein-coupled receptor; GPR159; G-protein coupled receptor 159; G-protein coupled receptor RDC1 homolog; RDC1; RDC-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP25106
  • Protein Name
    Atypical chemokine receptor 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GPCR RDC1/CXCR-7/ACKR3 using anti-GPCR RDC1/CXCR-7/ACKR3 antibody (A02656-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPCR RDC1/CXCR-7/ACKR3 antigen affinity purified polyclonal antibody (Catalog # A02656-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GPCR RDC1/CXCR-7/ACKR3 at approximately 41 kDa. The expected band size for GPCR RDC1/CXCR-7/ACKR3 is at 41 kDa.
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