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Anti-Aconitase 2 Antibody (Biotin)

Anti-Aconitase 2 Antibody (Biotin)

A84776
Antibodies.com
ApplicationsWestern Blot, ELISA
Product group Antibodies
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-ACO2 Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    Biotin
  • Host
    Goat
  • Isotype
    IgG
  • Scientific Description
    Goat polyclonal antibody to Aconitase 2 (Biotin).
  • UNSPSC
    12352203

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The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CSB-PA073168(ACO2 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)
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Figure 1. Western blot analysis of ACO2 using anti-ACO2 antibody (PB9973). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U87 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat skeletal muscle tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACO2 antigen affinity purified polyclonal antibody (Catalog # PB9973) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACO2 at approximately 85 kDa. The expected band size for ACO2 is at 85 kDa.
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