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Immunohistochemical staining of human cerebral cortex shows moderate granular cytoplasmic positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows moderate granular cytoplasmic positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows moderate granular cytoplasmic positivity in neurons.

Anti-ACSL4 Antibody

HPA005552
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetACSL4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ACSL4
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2182
  • Target name
    ACSL4
  • Target description
    acyl-CoA synthetase long chain family member 4
  • Target synonyms
    ACS4, FACL4, LACS4, MRX63, MRX68, XLID63, long-chain-fatty-acid--CoA ligase 4, acyl-CoA synthetase 4, arachidonate--CoA ligase, fatty-acid-Coenzyme A ligase, long-chain 4, lignoceroyl-CoA synthase, long-chain acyl-CoA synthetase 4, long-chain fatty-acid-Coenzyme A ligase 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60488
  • Protein Name
    Long-chain-fatty-acid--CoA ligase 4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of FACL4/ACSL4 using anti-FACL4/ACSL4 antibody (A04372-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FACL4/ACSL4 antigen affinity purified polyclonal antibody (Catalog # A04372-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FACL4/ACSL4 at approximately 79 kDa. The expected band size for FACL4/ACSL4 is at 79 kDa.
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