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ProductsBack/Goat anti-FACL4 / ACSL4

Goat anti-FACL4 / ACSL4

EB05662
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetACSL4
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-FACL4 / ACSL4 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the Golgi and Endoplasmic reticulum of HeLa cells. Recommended concentration: 10microg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC, IF, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID2182
  • Target name
    ACSL4
  • Target description
    acyl-CoA synthetase long chain family member 4
  • Target synonyms
    ACS4; acyl-CoA synthetase 4; arachidonate--CoA ligase; FACL4; fatty-acid-Coenzyme A ligase, long-chain 4; LACS4; lignoceroyl-CoA synthase; long-chain acyl-CoA synthetase 4; long-chain fatty-acid-Coenzyme A ligase 4; long-chain-fatty-acid--CoA ligase 4; MRX63; MRX68; XLID63
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_004449.1; NP_075266.1. GeneIDs all Nonhuman: 50790 (mouse); 113976 (rat);. Purification: Antigen affinity purified. Names and symbols: ACSL4; acyl-CoA synthetase long-chain family member 4; FACL4; fatty-acid-Coenzyme A ligase, long-chain 4; ACS4; LACS 4; lignoceroyl-CoA synthase; Fatty acid coenzyme A ligase, long-chain 4; MRX63; LACS4; MRX68; acyl-CoA synthetase 4; long-chain fatty-acid
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • A high-throughput pipeline for validation of antibodies. Sikorski K et al., 2018 Nov, Nat Methods
    Read more

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Figure 1. Western blot analysis of FACL4/ACSL4 using anti-FACL4/ACSL4 antibody (A04372-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FACL4/ACSL4 antigen affinity purified polyclonal antibody (Catalog # A04372-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FACL4/ACSL4 at approximately 79 kDa. The expected band size for FACL4/ACSL4 is at 79 kDa.
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