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Western blot All lanes: ACSL4 antibody at 2microg/ml Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 80, 75 kDa Observed band size: 80 kDa
Western blot All lanes: ACSL4 antibody at 2microg/ml Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 80, 75 kDa Observed band size: 80 kDa
Western blot All lanes: ACSL4 antibody at 2microg/ml Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 80, 75 kDa Observed band size: 80 kDa

ACSL4 Antibody

CSB-PA060488EA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetACSL4
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Overview

  • Supplier
    Cusabio
  • Product Name
    ACSL4 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2182
  • Target name
    ACSL4
  • Target description
    acyl-CoA synthetase long chain family member 4
  • Target synonyms
    ACS4, FACL4, LACS4, MRX63, MRX68, XLID63, long-chain-fatty-acid--CoA ligase 4, acyl-CoA synthetase 4, arachidonate--CoA ligase, fatty-acid-Coenzyme A ligase, long-chain 4, lignoceroyl-CoA synthase, long-chain acyl-CoA synthetase 4, long-chain fatty-acid-Coenzyme A ligase 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60488
  • Protein Name
    Long-chain-fatty-acid--CoA ligase 4
  • Scientific Description
    Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses arachidonate and eicosapentaenoate as substrates.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FACL4/ACSL4 using anti-FACL4/ACSL4 antibody (A04372-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FACL4/ACSL4 antigen affinity purified polyclonal antibody (Catalog # A04372-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FACL4/ACSL4 at approximately 79 kDa. The expected band size for FACL4/ACSL4 is at 79 kDa.
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