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Immunohistochemistry analysis in human lung and prostate tissues using AMAb91635 antibody. Corresponding AGER RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human lung and prostate tissues using AMAb91635 antibody. Corresponding AGER RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human lung and prostate tissues using AMAb91635 antibody. Corresponding AGER RNA-seq data are presented for the same tissues.

Anti-AGER Antibody

AMAB91635
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetAGER
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-AGER
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL10591
  • Conjugate
    Unconjugated
  • Gene ID177
  • Target name
    AGER
  • Target description
    advanced glycosylation end-product specific receptor
  • Target synonyms
    advanced glycosylation end product-specific receptor; RAGE; receptor for advanced glycation end-products variant 20; SCARJ1; sRAGE
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ15109
  • Protein Name
    Advanced glycosylation end product-specific receptor
  • Scientific Description
    Synthetic Peptide
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RAGE/AGER using anti-RAGE/AGER antibody (A03438-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAGE/AGER antigen affinity purified polyclonal antibody (Catalog # A03438-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAGE/AGER at approximately 45-58 kDa. The expected band size for RAGE/AGER is at 43 kDa.
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