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Immunohistochemical staining of human gastrointestinal shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human gastrointestinal shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human gastrointestinal shows strong granular cytoplasmic positivity in glandular cells.

Anti-ALDH18A1 Antibody

HPA012604
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetALDH18A1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ALDH18A1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5832
  • Target name
    ALDH18A1
  • Target description
    aldehyde dehydrogenase 18 family member A1
  • Target synonyms
    ADCL3, ARCL3A, GSAS, P5CS, PYCS, SPG9, SPG9A, SPG9B, delta-1-pyrroline-5-carboxylate synthase, Spastic paraplegia-9 (spastic paraparesis with amyotrophy, cataracts and gastroesophageal reflux), aldehyde dehydrogenase family 18 member A1, delta-1-pyrroline-5-carboxylate synthetase, delta1-pyrroline-5-carboxlate synthetase, pyrroline-5-carboxylate synthetase (glutamate gamma-semialdehyde synthetase), spastic paraplegia 9 (autosomal dominant)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP54886
  • Protein Name
    Delta-1-pyrroline-5-carboxylate synthase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of ALDH18A1 using anti-ALDH18A1 antibody (A06119-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MDA-MB-453 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse skeletal muscle tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH18A1 antigen affinity purified polyclonal antibody (Catalog # A06119-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALDH18A1 at approximately 87KD. The expected band size for ALDH18A1 is at 87KD.
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