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Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.
Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.
Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.

Anti-ALOX12 Antibody

HPA010691
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetALOX12
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ALOX12
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID239
  • Target name
    ALOX12
  • Target description
    arachidonate 12-lipoxygenase, 12S type
  • Target synonyms
    12-LOX; 12S-lipoxygenase; 12S-LOX; arachidonate (12S)-lipoxygenase; arachidonate (15S)-lipoxygenase; Arachidonate 12-lipoxygenase, 12S-type; arachidonate 15-lipoxygenase,15S-type; linoleate 13S-lipoxygenase; lipoxin synthase 12-LO; LOG12; platelet 12-LOX; platelet-type 12-lipoxygenase; platelet-type lipoxygenase 12; polyunsaturated fatty acid lipoxygenase ALOX12
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP18054
  • Protein Name
    Arachidonate 12-lipoxygenase, 12S-type
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Western Blot Positive WB detected in: K562 whole cell lysate, SH-SY5Y whole cell lysate All lanes: ALOX12 antibody at 3.53microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 76 kDa Observed band size: 76 kDa
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Figure 1. Western blot analysis of 12 Lipoxygenase using anti-12 Lipoxygenase antibody (A02275-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: rat spleen tissue lysates, lane 2: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-12 Lipoxygenase antigen affinity purified polyclonal antibody (Catalog # A02275-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 12 Lipoxygenase at approximately 80KD. The expected band size for 12 Lipoxygenase is at 75-80KD.
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