Anti-alpha 5 beta 1 Integrin [M200 (Volociximab)]

Absolute Antibody

Anti-alpha 5 beta 1 Integrin [M200 (Volociximab)]

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This antibody binds human alpha5beta1 integrin with high affinity (Kd = 0.367 nM, EC50 = 0.2 nM, IC50 = 2.3 nM) and, in chimeric mouse-human IgG4 form, demonstrated potent anti angiogenic and anti tumor effects in preclinical models (laser induced CNV in cynomolgus monkeys, VX2 carcinoma in rabbits) before progressing into Phase II trials in platinum resistant ovarian cancer (Ramakrishnan et al., 2006, PMID: 17024968; Bhaskar et al., 2007, PMID: 17786386). Exclusive binding to alpha5beta1 (no cross reactivity with alpha3beta1, alphavbeta3, alphavbeta5) was confirmed by ELISA. Immunohistochemistry (IHC-F) demonstrated binding to cynomolgus vasculature but not to mouse tissue. Studies with recombinant integrin subunits revealed that binding depends specifically on the human alpha5 chain, while the beta1 chain can be of either human or mouse origin, indicating strict specificity for the alpha5beta1 heterodimer (Bukowski et al., 2009; DOI: 10.1007/978-1-59745-332-5). In clinical testing, weekly 15 mg/kg IV dosing achieved trough serum levels >150 microg/mL, with 2-3x accumulation by Day 50, detectable antibody in ascites, reduction in circulating endothelial progenitor cells, and a favorable safety profile. The use of this antibody in IHC-P was demostrated (Bell McGuinn et al., 2011; PMID: 21276608). Clone F200, a Fab derivative of M200, is available upon request.

Flow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking

ELISA; FC; IF; Block; IHC

Research Use Only

Monoclonal

M200 (Volociximab)

ITGA5

integrin subunit alpha 5

CD49e, FNRA, VLA-5, VLA5A, integrin alpha-5, CD49 antigen-like family member E, ITGA5/SLC9B1 fusion, fibronectin receptor subunit alpha, fibronectin receptor, alpha polypeptide, fibronectin receptor, alpha subunit, integrin alpha-F, integrin, alpha 5 (fibronectin receptor, alpha polypeptide), very late activation protein 5, alpha subunit

Mouse

IgG1

Integrin alpha-5

NOT FOR THERAPEUTIC USE - This is a research-grade biosimilar. This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG4 format, for improved compatibility with existing reagents, assays and techniques.

Human

Human

Heterodimeric alfa5bèta1Fc fusion protein

-20°C,2°C to 8°C

41116161