Mouse anti Integrin alpha 5 (CD49e)

Nordic-MUbio

Mouse anti Integrin alpha 5 (CD49e)

7

NKI-SAM-1 recognizes the 135-kDa integrin- alpha 5 monocyte-specific antigen. As a result NKI-SAM-1 strongly reacts with monocytes, and weakly with granulocytes, platelets and T-lymphocytes. Its exact epitope is not known.

NKI-SAM-1 is suitable for flow cytometry (recommended range is 1:200 - 1:500, using 0.2-0.5 microg for 10^6 cells) and can be used to determine the VLA-5 expression in peripheral blood monocytes and monocyte derived dendritic cells (see refs 2 and 3), B cells and B-cell precursors (ref. 4), and in the less pathogenic yeast Candida species (ref. 5). NKI-SAM-1 can also be applied in immunohisto-chemistry on frozen tissues, immunocytochemistry and immunoprecipitation. Optimal antibody dilution should be determined by titration.

Flow Cytometry, ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen

Flow Cytometry;Immunohistochemistry (frozen);Immunoprecipitation;Immunocytochemistry

Primary antibodies

Research Use Only

Monoclonal

NKI-SAM-1

ITGA5

integrin subunit alpha 5

CD49 antigen-like family member E; CD49e; fibronectin receptor subunit alpha; fibronectin receptor, alpha polypeptide; fibronectin receptor, alpha subunit; FNRA; integrin alpha-5; integrin alpha-F; integrin, alpha 5 (fibronectin receptor, alpha polypeptide); ITGA5/SLC9B1 fusion; very late activation protein 5, alpha subunit; VLA-5; VLA5A

Mouse

IgG2b

Integrin alpha-5

NKI-SAM-1 is a monoclonal antibody obtained by fusion of mouse myeloma cells with spleen cells from BALB/C mice immunized with U937 histiocytic lymphoma cells.

Human, Primate

Human;macaque

12352203