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Anti-Alpha actin 1 [3G8C7A6]

Ab03147-10.0
Absolute Antibody
ApplicationsELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetACTA1
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Alpha actin 1 [3G8C7A6]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody is highly similar to the commercially available anti-actin antibody HHF35. This antibody can use used in the immunohistochemical staining of smooth fibroids. This antibody can be used in the identification of actin from various tissues including brain, heart, cerebellum, esophageal, adrenal, stomach, ovaries, small intestines, pancreatic, colorectal, parathyroid, liver, pituitary, salivary gland, testicular, kidney, thyroid, prostate, breast, uterus, spleen, bladder, tonsil, skeletal muscle, thymus (young children), skin, bone marrow, peripheral nerve, lung, melodder cells. The binding specificity of this antibody towards actin was determined using ELISA. The original IgG2b antibody binds actin with an affinity constant of 6.26X10^9 M^(-1) (CN113817055).
  • Applications
    ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA; IHC-P
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3G8C7A6
  • Gene ID58
  • Target name
    ACTA1
  • Target description
    actin alpha 1, skeletal muscle
  • Target synonyms
    ACTA; actin, alpha skeletal muscle; ASMA; CFTD; CFTD1; CFTDM; MPFD; NEM1; NEM2; NEM3; nemaline myopathy type 3; SHPM
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDP68133
  • Protein Name
    Actin, alpha skeletal muscle
  • Scientific Description
    This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    The original antibody was generated by immunizing BALB/c mice with a recombinant human actin protein.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Actin using anti-Actin antibody (PB9751). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse heart tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse spleen tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: human Hela whole cell lysates, Lane 9: human HepG2 whole cell lysates, Lane 10: human 293T whole cell lysates, Lane 11: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Actin antigen affinity purified polyclonal antibody (Catalog # PB9751) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Actin at approximately 42 kDa. The expected band size for Actin is at 42 kDa.
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