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Mouse anti actin alpha-skeletal

MUB0108P
Nordic-MUbio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetACTA1
0.1 mg
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti actin alpha-skeletal
  • Delivery Days Customer
    7
  • Application Supplier Note
    3B3 is useful for immunohistochemistry on frozen and paraffin-embedded tissues, immunoblotting and ELISA. Optimal antibody dilutions should be determined by titration; recommended range is 1:100 - 1:1000 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:1000 - 1:5000 for immunoblotting applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA;Immunohistochemistry (frozen);Immunohistochemistry (paraffin);Western Blotting
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3B3
  • Gene ID58
  • Target name
    ACTA1
  • Target description
    actin alpha 1, skeletal muscle
  • Target synonyms
    ACTA; actin, alpha skeletal muscle; ASMA; CFTD; CFTD1; CFTDM; MPFD; NEM1; NEM2; NEM3; nemaline myopathy type 3; SHPM
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP68133
  • Protein Name
    Actin, alpha skeletal muscle
  • Source
    3B3 is a Mouse monoclonal IgG1 antibody derived by fusion of NS0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a peptide comprising the N-terminal nonapeptide of alpha-skeletal actin with an acetylated N-terminus ...
  • Reactivity Supplier
    Caprine;Human;Rabbit;Rat;Swine
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Actin using anti-Actin antibody (PB9751). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse heart tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse spleen tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: human Hela whole cell lysates, Lane 9: human HepG2 whole cell lysates, Lane 10: human 293T whole cell lysates, Lane 11: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Actin antigen affinity purified polyclonal antibody (Catalog # PB9751) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Actin at approximately 42 kDa. The expected band size for Actin is at 42 kDa.
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