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Western Blot analysis of K562 cells using FTO Polyclonal Antibody
Western Blot analysis of K562 cells using FTO Polyclonal Antibody
Western Blot analysis of K562 cells using FTO Polyclonal Antibody

FTO Antibody

CSB-PA004640
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetFTO
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Overview

  • Supplier
    Cusabio
  • Product Name
    FTO Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79068
  • Target name
    FTO
  • Target description
    FTO alpha-ketoglutarate dependent dioxygenase
  • Target synonyms
    ALKBH9, BMIQ14, GDFD, IFEX9, alpha-ketoglutarate-dependent dioxygenase FTO, AlkB homolog 9, U6 small nuclear RNA (2'-O-methyladenosine-N(6)-)-demethylase FTO, U6 small nuclear RNA N(6)-methyladenosine-demethylase FTO, fat mass and obesity associated, fat mass and obesity-associated protein, intragenic FTO exon 9 containing transcript, m6A(m)-demethylase FTO, mRNA (2'-O-methyladenosine-N(6)-)-demethylase FTO, mRNA N(6)-methyladenosine demethylase FTO, tRNA N1-methyl adenine demethylase FTO
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9C0B1
  • Protein Name
    Alpha-ketoglutarate-dependent dioxygenase FTO
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FTO using anti-FTO antibody (A00219-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FTO antigen affinity purified polyclonal antibody (Catalog # A00219-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FTO at approximately 58 kDa. The expected band size for FTO is at 58 kDa.
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