Figure 1. Western blot analysis of SGCA using anti-SGCA antibody (PB9503). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Skeletal Muscle Tissue Lysate at 50ug, Lane 2: Mouse Skeletal Muscle Tissue Lysate at 50ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SGCA antigen affinity purified polyclonal antibody (Catalog # PB9503) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SGCA at approximately 43 kDa. The expected band size for SGCA is at 43 kDa.
Figure 1. Western blot analysis of SGCA using anti-SGCA antibody (PB9503). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Skeletal Muscle Tissue Lysate at 50ug, Lane 2: Mouse Skeletal Muscle Tissue Lysate at 50ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SGCA antigen affinity purified polyclonal antibody (Catalog # PB9503) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SGCA at approximately 43 kDa. The expected band size for SGCA is at 43 kDa.
Anti-alpha Sarcoglycan/SGCA Antibody Picoband(r)
PB9503-HRP
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSGCA
Overview
- SupplierBoster Bio
- Product NameAnti-alpha Sarcoglycan/SGCA Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateHRP
- Gene ID6442
- Target nameSGCA
- Target descriptionsarcoglycan alpha
- Target synonyms50 kDa dystrophin-associated glycoprotein; 50DAG; 50kD DAG; adhalin; ADL; alpha-sarcoglycan; alpha-SG; DAG2; DMDA2; dystroglycan-2; LGMD2D; LGMDR3; limb girdle muscular dystrophy 2D; sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein); SCARMD1
- HostRabbit
- IsotypeIgG
- Protein IDQ16586
- Protein NameAlpha-sarcoglycan
- Scientific DescriptionBoster Bio Anti-alpha Sarcoglycan/SGCA Antibody Picoband® catalog # PB9503. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203