Immunohistochemical analysis of paraffin-embedded human colon, using alpha smooth muscle Actin Antibody(M01072-1) ACTA2 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ACTA2 Antibody (M01072-1)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Immunohistochemical analysis of paraffin-embedded human colon, using alpha smooth muscle Actin Antibody(M01072-1) ACTA2 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ACTA2 Antibody (M01072-1)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody
M01072-1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetACTA2
Overview
- SupplierBoster Bio
- Product NameAnti-alpha smooth muscle Actin Rabbit Monoclonal Antibody
- Delivery Days Customer9
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDEO-1
- FormulationLiquid
- Gene ID59
- Target nameACTA2
- Target descriptionactin alpha 2, smooth muscle
- Target synonymsactin, alpha 2, smooth muscle, aorta; actin, aortic smooth muscle; ACTSA; alpha-cardiac actin; cell growth-inhibiting gene 46 protein
- HostRabbit
- IsotypeIgG
- Protein IDP62736
- Protein NameActin, aortic smooth muscle
- Scientific DescriptionBoster Bio Anti-alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody catalog # M01072-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203
References
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- Increased expression of chitinase 3-like 1 in aorta of patients with atherosclerosis and suppression of atherosclerosis in apolipoprotein E-knockout mice by chitinase 3-like 1 gene silencing. Gong Z et al., 2014, Mediators InflammRead more