Mouse anti actin alpha-muscle

Nordic-MUbio

Mouse anti actin alpha-muscle

7

HHF35 reacts with both alpha-muscle and gamma-smooth muscle actin, and therefore reacts with skeletal muscle, cardiac muscle, vascular and visceral smooth muscle cells, pericytes and myoepithelial cells. It is also reactive in myofibroblasts. It does not

HHF35 is useful for immunohistochemistry on frozen and paraffin-embedded tissues preserved in several types of fixatives (see reference 1), immunoblotting, immuno-electron microscopy and ELISA. For immunohistochemical applications to paraffin embedded tissues it is recommended to dilute the antibody in PBS containing 50 mM EDTA. Optimal antibody dilution should be determined by titration; recommended range is 1:200 - 1:1000 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:1000 - 1:5000 for immunoblotting applications.

Electron Microscopy, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Electron microscopy;ELISA;Immunocytochemistry;Immunohistochemistry (frozen);Immunohistochemistry (paraffin);Western Blotting

Primary antibodies

Research Use Only

Monoclonal

HHF35

ACTA2

actin alpha 2, smooth muscle

actin, alpha 2, smooth muscle, aorta; actin, aortic smooth muscle; ACTSA; alpha-cardiac actin; cell growth-inhibiting gene 46 protein

Mouse

IgG1

Actin, aortic smooth muscle

HHF35 is a Mouse monoclonal IgG1 antibody derived by fusion of NS-1 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with an SDS-extracted protein fraction from Human myocardium.

Chicken, Human, Monkey, Porcine, Rabbit, Rat, Zebra Fish

Chicken;Human;Monkey;Rabbit;Rat;Swine;Zebrafish

12352203