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AMACR antibody

FNAB00355
Wuhan Fine Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetAMACR
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Overview

  • Supplier
    Wuhan Fine Biotech
  • Product Name
    AMACR antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Estimated Purity
    ≥95%
  • Gene ID23600
  • Target name
    AMACR
  • Target description
    alpha-methylacyl-CoA racemase
  • Target synonyms
    AMACRD, CBAS4, P504S, RACE, RM, alpha-methylacyl-CoA racemase, 2-methylacyl-CoA racemase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UHK6
  • Protein Name
    Alpha-methylacyl-CoA racemase
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AMHR2 using anti-AMHR2 antibody (PB9983). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates. Lane 2: rat liver tissue lysates. Lane 3: HEPG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMHR2 antigen affinity purified polyclonal antibody (Catalog # PB9983) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002)?with Tanon 5200 system. A specific band was detected for AMHR2 at approximately 42 kDa. The expected band size for AMHR2 is at 42 kDa.
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