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Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity.
Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity.
Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity.

Anti-AMPD1 Antibody

HPA026478
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetAMPD1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-AMPD1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID270
  • Target name
    AMPD1
  • Target description
    adenosine monophosphate deaminase 1
  • Target synonyms
    adenosine monophosphate deaminase 1 (isoform M); adenosine monophosphate deaminase-1 (muscle); AMP deaminase 1; AMPD; MAD; MADA; MMDD; myoadenylate deaminase; skeletal muscle AMPD
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP23109
  • Protein Name
    AMP deaminase 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: MCF-7 whole cell lysate, PC-3 whole cell lysate, A549 whole cell lysate All lanes: AMPD1 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 91, 90 kDa Observed band size: 91 kDa
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Figure 1. Western blot analysis of AMPD1 using anti-AMPD1 antibody (A04209-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: rat skeletal muscle tissue lysates, Lane 5: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMPD1 antigen affinity purified polyclonal antibody (Catalog # A04209-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMPD1 at approximately 86 kDa. The expected band size for AMPD1 is at 86 kDa.
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