anti-Apaf-1, mAb (18H2)

AdipoGen Life Sciences

anti-Apaf-1, mAb (18H2)

10

Recognizes the CARD domain of human and mouse Apaf-1. Does not cross-react with rat Apaf-1.

ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry

Research Use Only

Monoclonal

18H2

1 mg/ml

>95%

Apaf1

apoptotic peptidase activating factor 1

6230400I06Rik; Ap; Apaf-1; Apaf1l; apoptotic protease-activating factor 1; fog; forebrain overgrowth; mKIAA0413

Rat

IgG2a

Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. - Monoclonal Antibody. Recognizes the CARD domain of human and mouse Apaf-1. Does not cross-react with rat Apaf-1. Isotype: Rat IgG2akappa. Clone: 18H2. Applications: ELISA, ICC, IP, WB. Liquid. In PBS containing 0.02% sodium azide. Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.

Human, Mouse

2°C to 8°C,-20°C

12352203