Western blot analysis using anti-Apaf-1 (mouse/rat), MAb (13F11) (AG-20T-0133) on purified cells from mouse tissues. The ~130kDa band corresponds to endogenous Apaf-1, which is absent in cells from Apaf-1 KO mice.
Western blot analysis using anti-Apaf-1 (mouse/rat), MAb (13F11) (AG-20T-0133) on purified cells from mouse tissues. The ~130kDa band corresponds to endogenous Apaf-1, which is absent in cells from Apaf-1 KO mice.
anti-Apaf-1 (mouse/rat), mAb (13F11)
AG-20T-0133
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityMouse, Rat
TargetApaf1
Overview
- SupplierAdipoGen Life Sciences
- Product Nameanti-Apaf-1 (mouse/rat), mAb (13F11)
- Delivery Days Customer10
- Antibody SpecificityRecognizes the CARD domain of mouse and rat Apaf-1. Does not cross-react with human Apaf-1.
- ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID13F11
- Concentration1 mg/ml
- Estimated Purity>95%
- Gene ID78963
- Target nameApaf1
- Target descriptionapoptotic peptidase activating factor 1
- Target synonymsapaf-1; apoptotic protease-activating factor 1
- HostRat
- IsotypeIgG2a
- Scientific DescriptionApaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. - Monoclonal Antibody. Recognizes the CARD domain of mouse and rat Apaf-1. Does not cross-react with human Apaf-1. Isotype: Rat IgG2akappa. Clone: 13F11. Applications: ELISA, ICC, IP, WB. Liquid. In PBS containing 0.02% sodium azide. Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.
- ReactivityMouse, Rat
- Storage Instruction2°C to 8°C,-20°C
- UNSPSC12352203