anti-Apaf-1 (human), mAb (2E12)

AdipoGen Life Sciences

anti-Apaf-1 (human), mAb (2E12)

10

ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry

Research Use Only

Monoclonal

2E12

1 mg/ml

>95%

APAF1

apoptotic peptidase activating factor 1

APAF-1; apoptotic protease-activating factor 1; CED4

Rat

IgG2a

Apoptotic protease-activating factor 1

Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. - Monoclonal Antibody. Recognizes the CARD domain of human Apaf-1. Does not cross-react with mouse or rat Apaf-1. Isotype: Rat IgG2akappa. Clone: 2.00E+12. Applications: ELISA, ICC, IHC, IP, WB. Liquid. In PBS containing 0.02% sodium azide. Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.

-20°C,2°C to 8°C

12352203