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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus, nucleoli fibrillar center & vesicles.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus, nucleoli fibrillar center & vesicles.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus, nucleoli fibrillar center & vesicles.

Anti-APEX2 Antibody

HPA048577
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetAPEX2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-APEX2 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID27301
  • Target name
    APEX2
  • Target description
    apurinic/apyrimidinic endodeoxyribonuclease 2
  • Target synonyms
    APE2, APEXL2, XTH2, ZGRF2, DNA-(apurinic or apyrimidinic site) endonuclease 2, AP endonuclease 2, AP endonuclease XTH2, APEX nuclease (apurinic/apyrimidinic endonuclease) 2, DNA-(apurinic or apyrimidinic site) lyase 2, apurinic/apyrimidinic endonuclease-like 2, zinc finger, GRF-type containing 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UBZ4
  • Protein Name
    DNA-(apurinic or apyrimidinic site) endonuclease 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of APEX2 using anti-APEX2 antibody (A07203-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APEX2 antigen affinity purified polyclonal antibody (Catalog # A07203-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APEX2 at approximately 57 kDa. The expected band size for APEX2 is at 57 kDa.
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