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Formalin-fixed and paraffin embedded rat brain labeled with Anti-APEX2 Polyclonal Antibody, Unconjugated (bs-6587R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Anti-APEX2 Polyclonal Antibody, Unconjugated (bs-6587R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Anti-APEX2 Polyclonal Antibody, Unconjugated (bs-6587R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

APEX2 Polyclonal Antibody

BS-6587R
Bioss
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetAPEX2
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Overview

  • Supplier
    Bioss
  • Product Name
    APEX2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID27301
  • Target name
    APEX2
  • Target description
    apurinic/apyrimidinic endodeoxyribonuclease 2
  • Target synonyms
    APE2, APEXL2, XTH2, ZGRF2, DNA-(apurinic or apyrimidinic site) endonuclease 2, AP endonuclease 2, AP endonuclease XTH2, APEX nuclease (apurinic/apyrimidinic endonuclease) 2, DNA-(apurinic or apyrimidinic site) lyase 2, apurinic/apyrimidinic endonuclease-like 2, zinc finger, GRF-type containing 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UBZ4
  • Protein Name
    DNA-(apurinic or apyrimidinic site) endonuclease 2
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of APEX2 using anti-APEX2 antibody (A07203-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APEX2 antigen affinity purified polyclonal antibody (Catalog # A07203-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APEX2 at approximately 57 kDa. The expected band size for APEX2 is at 57 kDa.
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