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Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.

Anti-ARHGAP26 Antibody

HPA035106
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetARHGAP26
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ARHGAP26
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23092
  • Target name
    ARHGAP26
  • Target description
    Rho GTPase activating protein 26
  • Target synonyms
    GRAF; GRAF1; GTPase regulator associated with focal adhesion kinase pp125(FAK); oligophrenin-1-like protein; OPHN1L; OPHN1L1; rho GTPase-activating protein 26
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UNA1
  • Protein Name
    Rho GTPase-activating protein 26
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GRAF/ARHGAP26 using anti-GRAF/ARHGAP26 antibody (A08027-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRAF/ARHGAP26 antigen affinity purified polyclonal antibody (Catalog # A08027-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRAF/ARHGAP26 at approximately 100 kDa. The expected band size for GRAF/ARHGAP26 is at 92 kDa.
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