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ProductsBack/ARHGAP26 / GRAF Antibody (aa625-814)

ARHGAP26 / GRAF Antibody (aa625-814)

LS-C380287
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetARHGAP26
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ARHGAP26 / GRAF Antibody (aa625-814)
  • Delivery Days Customer
    23
  • Applications
    ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, IHC, IHC-P (1:20 - 1:200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID23092
  • Target name
    ARHGAP26
  • Target description
    Rho GTPase activating protein 26
  • Target synonyms
    GRAF; GRAF1; GTPase regulator associated with focal adhesion kinase pp125(FAK); oligophrenin-1-like protein; OPHN1L; OPHN1L1; rho GTPase-activating protein 26
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GRAF/ARHGAP26 using anti-GRAF/ARHGAP26 antibody (A08027-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRAF/ARHGAP26 antigen affinity purified polyclonal antibody (Catalog # A08027-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRAF/ARHGAP26 at approximately 100 kDa. The expected band size for GRAF/ARHGAP26 is at 92 kDa.
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