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Immunohistochemical staining of human cerebral cortex shows weak to moderate cytoplasmic positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows weak to moderate cytoplasmic positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows weak to moderate cytoplasmic positivity in neurons.

Anti-ARHGEF7 Antibody

HPA004744
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetARHGEF7
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ARHGEF7
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8874
  • Target name
    ARHGEF7
  • Target description
    Rho guanine nucleotide exchange factor 7
  • Target synonyms
    BETA-PIX, COOL-1, COOL1, Nbla10314, P50, P50BP, P85, P85COOL1, P85SPR, PAK3, PIXB, rho guanine nucleotide exchange factor 7, PAK-interacting exchange factor beta, Rho guanine nucleotide exchange factor (GEF) 7, SH3 domain-containing proline-rich protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14155
  • Protein Name
    Rho guanine nucleotide exchange factor 7
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of ARHGEF7 using anti-ARHGEF7 antibody (A02764-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse skeletal muscle lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARHGEF7 antigen affinity purified polyclonal antibody (Catalog # A02764-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARHGEF7 at approximately 80 kDa. The expected band size for ARHGEF7 is at 90 kDa.
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Immunofluorescence staining of Hela cells with CSB-PA01445A0Rb at 1:250, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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