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Arhgef7 Polyclonal Antibody

CAC08631
Biomatik
ApplicationsImmunoFluorescence, ImmunoPrecipitation, ELISA
Product group Antibodies
TargetARHGEF7
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Overview

  • Supplier
    Biomatik
  • Product Name
    ARHGEF7 Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, ELISA
  • Applications Supplier
    ELISA, IF, IP; Recommended dilution: IF:1:200-1:500, IP:1:200-1:2000
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8874
  • Target name
    ARHGEF7
  • Target description
    Rho guanine nucleotide exchange factor 7
  • Target synonyms
    BETA-PIX, COOL-1, COOL1, Nbla10314, P50, P50BP, P85, P85COOL1, P85SPR, PAK3, PIXB, rho guanine nucleotide exchange factor 7, PAK-interacting exchange factor beta, Rho guanine nucleotide exchange factor (GEF) 7, SH3 domain-containing proline-rich protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14155
  • Protein Name
    Rho guanine nucleotide exchange factor 7
  • Scientific Description
    The Arhgef7 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, IF, IP.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ARHGEF7 using anti-ARHGEF7 antibody (A02764-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse skeletal muscle lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARHGEF7 antigen affinity purified polyclonal antibody (Catalog # A02764-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARHGEF7 at approximately 80 kDa. The expected band size for ARHGEF7 is at 90 kDa.
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