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ProductsBack/Anti-Aspartate beta-hydroxylase [G3-F11]

Anti-Aspartate beta-hydroxylase [G3-F11]

AB03472-1.1-BT
Absolute Antibody
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetASPH
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Aspartate beta-hydroxylase [G3-F11]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The specificity and sensitivity of this antibody for human aspartyl (asparaginyl) beta-hydroxylase (HAAH) was determined using ELISA and western blot (PMID: 19663697). This antibody was also used in the identification of HAAH expression in seven tumor tissues, including hepatocellular carcinoma, lung cancer, kidney cancer, cholangiocarcinoma, prostate cancer, breast cancer, and glioblastoma by immunohistochemical stain (PMID: 19663697; 25394783).
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    G3-F11
  • Gene ID444
  • Target name
    ASPH
  • Target description
    aspartate beta-hydroxylase
  • Target synonyms
    A beta H-J-J; AAH; ASP beta-hydroxylase; aspartyl/asparaginyl beta-hydroxylase; BAH; cardiac junctin; CASQ2BP1; FDLAB; HAAH; humbug; JCTN; junctate; junctin; peptide-aspartate beta-dioxygenase
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ12797
  • Protein Name
    Aspartyl/asparaginyl beta-hydroxylase
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ASPH using anti-ASPH antibody (PB9478). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate, Lane 2: Rat Liver Tissue Lysate, Lane 3: HELA Whole Cell Lysate, Lane 4: HEPG2 Whole Cell Lysate, Lane 5: HEPA Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASPH antigen affinity purified polyclonal antibody (Catalog # PB9478) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASPH at approximately 100KD. The expected band size for ASPH is at 86KD.
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