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Western Blot Positive WB detected in: A549 whole cell lysate, HepG2 whole cell lysate, Mouse kidney tissue All lanes: ASPH antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 86, 35, 26, 24, 34, 84, 30, 22, 29, 33 kDa Observed band size: 86 kDa
Western Blot Positive WB detected in: A549 whole cell lysate, HepG2 whole cell lysate, Mouse kidney tissue All lanes: ASPH antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 86, 35, 26, 24, 34, 84, 30, 22, 29, 33 kDa Observed band size: 86 kDa
Western Blot Positive WB detected in: A549 whole cell lysate, HepG2 whole cell lysate, Mouse kidney tissue All lanes: ASPH antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 86, 35, 26, 24, 34, 84, 30, 22, 29, 33 kDa Observed band size: 86 kDa

ASPH Antibody

CSB-PA002226HA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetASPH
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Overview

  • Supplier
    Cusabio
  • Product Name
    ASPH Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID444
  • Target name
    ASPH
  • Target description
    aspartate beta-hydroxylase
  • Target synonyms
    A beta H-J-J; AAH; ASP beta-hydroxylase; aspartyl/asparaginyl beta-hydroxylase; BAH; cardiac junctin; CASQ2BP1; FDLAB; HAAH; humbug; JCTN; junctate; junctin; peptide-aspartate beta-dioxygenase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ12797
  • Protein Name
    Aspartyl/asparaginyl beta-hydroxylase
  • Scientific Description
    Isoform 1:specifically hydroxylates an Asp or Asn residue in certain epidermal growth factor-like (EGF) domains of a number of proteins. Ref.13 Isoform 8:membrane-bound Ca2+-sensing protein, which is a structural component of the ER-plasma membrane junctions. Isoform 8 regulates the activity of Ca(+2) released-activated Ca(+2) (CRAC) channels in T-cells.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ASPH using anti-ASPH antibody (PB9478). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate, Lane 2: Rat Liver Tissue Lysate, Lane 3: HELA Whole Cell Lysate, Lane 4: HEPG2 Whole Cell Lysate, Lane 5: HEPA Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASPH antigen affinity purified polyclonal antibody (Catalog # PB9478) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASPH at approximately 100KD. The expected band size for ASPH is at 86KD.
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