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Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in astrocytes.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in astrocytes.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in astrocytes.

Anti-ASPH Antibody

HPA059303
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetASPH
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ASPH
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID444
  • Target name
    ASPH
  • Target description
    aspartate beta-hydroxylase
  • Target synonyms
    A beta H-J-J; AAH; ASP beta-hydroxylase; aspartyl/asparaginyl beta-hydroxylase; BAH; cardiac junctin; CASQ2BP1; FDLAB; HAAH; humbug; JCTN; junctate; junctin; peptide-aspartate beta-dioxygenase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ12797
  • Protein Name
    Aspartyl/asparaginyl beta-hydroxylase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Western Blot Positive WB detected in: A549 whole cell lysate, HepG2 whole cell lysate, Mouse kidney tissue All lanes: ASPH antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 86, 35, 26, 24, 34, 84, 30, 22, 29, 33 kDa Observed band size: 86 kDa
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Figure 1. Western blot analysis of ASPH using anti-ASPH antibody (PB9478). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate, Lane 2: Rat Liver Tissue Lysate, Lane 3: HELA Whole Cell Lysate, Lane 4: HEPG2 Whole Cell Lysate, Lane 5: HEPA Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASPH antigen affinity purified polyclonal antibody (Catalog # PB9478) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASPH at approximately 100KD. The expected band size for ASPH is at 86KD.
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